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KMID : 0352720000240030128
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Journal of Ginseng Research
2000 Volume.24 No. 3 p.128 ~ p.133
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Isolation and Characterization of Immunomodulatory Glycoprotein from the Root of Panax ginseng
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Jong Dae Park/Han Jae Shin
Kyeong Mee Park/Young Sook Kim/Ki Yeul Nam/You Hui Lee/Jong Dae Park
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Abstract
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A high molecular (more than 10 kDa) fraction, showing mitogenic and comitogenic activities in spleen cells of mouse, was isolated from water extract of ginseng. The crude protein substance prepared by 80% (NH4)2SO4 precipitation from this fraction was purified and isolated by DEAE Sepharose column chromatography. Among the fractions eluted, it was found that four kinds of fractions eluted with 0 to 1 M NaCl gradient were glycoproteins, which induced proliferation of spleen cells and increased NO production in macrophages. Among them, F-2 fraction, which contained 35.9% protein,49.4% neutral sugar and 12.5% uronic acid, was found to show mitogenic activity as strong as that of LPS (lipopolysaccharide) at a concentration of 100 μg/ml and to remarkably stimulate NO production by murine macrophages at a concentration of 500 μg/ml. When F-2 is deproteinized, the mitogenic activity of F-2 was decreased significantly to 70.9% as compared with that of F-2. This results suggests that the protein moiety of F-2 may play an important role in immunomodulating activity of glycoprotein from the root of Panax ginseng.
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KEYWORD
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Panax ginseng, glycoprotein, mitogenic activity, macrophage, nitric oxide,
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